The
Halorubrum lacusprofundi
Genome
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Hlac_2868
Gene ID
2868
Gene Name
Hlac_2868
Annotation
Beta Galactosidase Family 42
Location
pHL500:127597←129699
Gene type
Functional gene
GC content
0.666666666666667
Hydropathicity
-0.546857142857141
DNA (2103 bp,
Edit
)
>Hlac_2868|2868|127597|Rev|129699
ATGCGTCTCGGAGTCTGTTACTTTCCCGAACATTGGCCGAGCGAAGAGTGGGAACGCGATGTCGCTGCGATGGCCGACGC
CGGCCTCGAATACGTGCGGATGGCGGAGTTCTCGTGGGGAGTCCTCGAGCCCGAGCGGGGTACATTCGATTTCGAGTGGC
TTGATGAGGCCATCGAGCTCATCGGCGACCACGGCATGCAGGCGGTGTTGTGCACGCCGACGGCGACGCCGCCGAAGTGG
CTGGTCGACGAGCGGCCGTCGATCCGGCAAGAAGACCCCGACGGAACCGTCCGCGAACACGGCAGCCGCCGGCACTACTG
TTTCAACTCCGACGCCTACCGCGAGGAGACAGCTCGGATCGTCGAGCGGGTCACCGAGCGATACGCCGACTCGCCGCACG
TCGCCGGCTGGCAGACGGACAACGAGTTCGGCTGTCACGAGACCGTCCGGTGTTACTGCGACGATTGTGCCGACGCGTTC
CGAACGTGGCTCGCGGACCGGTACGGTGATATTGACCGACTCAACGAGGCGTGGGGGAACGCGTTCTGGAGCCAGCAGTA
CGGCTCCTTCGACGAAATCGATCCGCCTGGGCCGACCCCTGCAGAGCATCACCCCTCGCGGCTGCTGGCGTACGCACGCT
TTTCCAGCGATTCCGTCGTCGAGTACAATCGCCTCCACGCCGACCTGATACGCGAGGCTGACCCCGACTGGTTCGTCACC
CACAATTTCATGGGGCGGTTCCCGACGCTGAACGCCTACGACGTGAGCGAGGACCTCGACCGCGTCGCCTGGGACTCATA
CCCGACGGGGTTCGTCCAGGACCGATACGACGGGGAGGCATCCCCGGACCAGCTGCGAGCCGGCGACCCCGACCAGGTCG
GGATGGATCACGACATCTACCGGAGCGCGCTCGACCGGCCGTTCTGGGTGATGGAACAACAACCGGGCGATGTAAACTGG
CCGCCGCACTGTCCGCAGCCGGGTGAGGGTGCTATGCGTCTGTGGGCGCACCACGCCGCCGCCCACGGAGCCGACGCAGT
CCTCTACTTCAGGTGGCGGCGCTGTCTCGAGGGTCAAGAACAGTATCACGCCGGACTCCGGAAGGCCGACGGGTCGCCCG
ACCGCGGATACGCCGACGCCGCACACACGTCCGAGGAGTTCGCGACTCTCGACGGGGCCAGTCACGTGGACGCCCCGGTG
GCGGTTGTGTTCGACTATGACTCGCTCTGGGCGCTCAACGCGCAGCCGCACGCCCCGGACTTCGACTACTGGGCGCTCCA
AGAGGCATTCTACGGCGCTGTACGCGGCCGTGGCGTTCAGGTCGATGTGGTTCCGCCAAGTGCCGATCTCTCCGGATACG
CGGCCGTCGTCGCGCCCGCACTCCACCTCGTTACCGAGGATCTCGCCGACCGGCTGACCGACTACATCGCCGGCGGCGGG
GAGGTTCTCTTCGGTCCCCGGACCGGCGTGAAGGACGCAGAAAACAAGCTCCGGCCGATGTCCCAGCCCGGCCCGCTGAC
CGACCTCGTCGGCGCGACCGTCGATCAGCACGAGTCGCTGCCGCGGCGCCTCGAAACGACAGTCCGACGAGTTGGTGACC
CCACCGACGACTCCGAGGAGATCGCGGCGCCGCCCGTCTCGTTCCGAACCTGGGCGGAGTGGCTGGACCCCGATGCAGCC
GAGCCTCAGTACGCGTACGATGTCGACGGGCCGGCGGACGGTCGGCCGGCAGTCGTTACCAACACAGTCGGTGACGGGCA
AGTCACTTACTGTGGAGTCTGGCCCGAGTCTGACCTAGCGGACGCGCTTGCGTCAGACCTGCTCGACCGCGCGGGCGTCC
GATACGCCGAGCGGCTCCCAGACGGTGTCCGGATCGGGTACCGCGGCGGCCGCACGTGGGTGACGAACTTCACGAGCGAC
CGGCTCCGGTTGCCCGAAATCGATCCCGAGTCGCTGGCGGTTGACGACACTGATCGGGACGGTTTCGACCCGATGGCGGA
CGACGACAAGGACTCGTCTGCAGACGGTATTGTTGTGGGGCCGTACGGGGTCGCCGTCATCGAGGGTGACTGCGTGGACG
GGCTGAGAATCGCTCAGACGTAG
E.C.#
3.2.1.23
pI
4.1818
MW (k)
78056.6
Translation (701 aa,
Edit
)
>Hlac_2868|2868|gene !!AA_SEQUENCE 1.0
MRLGVCYFPEHWPSEEWERDVAAMADAGLEYVRMAEFSWGVLEPERGTFDFEWLDEAIELIGDHGMQAVLCTPTATPPKW
LVDERPSIRQEDPDGTVREHGSRRHYCFNSDAYREETARIVERVTERYADSPHVAGWQTDNEFGCHETVRCYCDDCADAF
RTWLADRYGDIDRLNEAWGNAFWSQQYGSFDEIDPPGPTPAEHHPSRLLAYARFSSDSVVEYNRLHADLIREADPDWFVT
HNFMGRFPTLNAYDVSEDLDRVAWDSYPTGFVQDRYDGEASPDQLRAGDPDQVGMDHDIYRSALDRPFWVMEQQPGDVNW
PPHCPQPGEGAMRLWAHHAAAHGADAVLYFRWRRCLEGQEQYHAGLRKADGSPDRGYADAAHTSEEFATLDGASHVDAPV
AVVFDYDSLWALNAQPHAPDFDYWALQEAFYGAVRGRGVQVDVVPPSADLSGYAAVVAPALHLVTEDLADRLTDYIAGGG
EVLFGPRTGVKDAENKLRPMSQPGPLTDLVGATVDQHESLPRRLETTVRRVGDPTDDSEEIAAPPVSFRTWAEWLDPDAA
EPQYAYDVDGPADGRPAVVTNTVGDGQVTYCGVWPESDLADALASDLLDRAGVRYAERLPDGVRIGYRGGRTWVTNFTSD
RLRLPEIDPESLAVDDTDRDGFDPMADDDKDSSADGIVVGPYGVAVIEGDCVDGLRIAQT
Set Format
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Phred
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tab-delimited
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Comment
Hlac_2868 codes for a family 42 beta-galactosidase enzyme. It was first investigated using bioinformatics to compare it to homologous haloarchaeal proteins. See DasSarma et al. [Plos One. 2013 Mar;8(3):e58587](https://www.ncbi.nlm.nih.gov/pubmed/23536799). The enzyme was then cloned, overexpressed, purified, and characterized using NRC-1 as a haloarchaeal expression system. See Karan et al. [BMC Biotech. 2013 Jan;13(3)](https://www.ncbi.nlm.nih.gov/pubmed/23320757). In order to determine the source of the enzyme’s cold adaptation the bioinformatic and genetic approaches were combined to perform site directed single amino acid mutations and it was determined that single amino acid changes can affect the cold adaptation of the enzyme. See Laye et al. [Proc Natl Acad Sci U S A. 2017 Oct;114(47):12530-5](https://www.ncbi.nlm.nih.gov/pubmed/29109294). Function of the enzyme with Martian perchlorate concentration was also tested. See Laye and DasSarma [Astrobiology. 2017 Nov 30. doi: 10.1089/ast.2017.1766](https://www.ncbi.nlm.nih.gov/pubmed/29189043).
